Hek293 atcc

T is a highly transfectable derivative of the 2cell line into which the temperature sensitive gene for SVT-antigen was inserted. Purchase HEK2(ATCC ) HEK2research articles. Production of P-glycoprotein: HEK 2cells were transfected to transiently express P-glycoprotein with a histidine tag to study the phenomenon of drug efflux. The P-glycoprotein was then purified with nickel-chelate chromatography and analyzed for drug binding and drug-stimulated ATPase activity. There are several ways to find pricing and availability for our products.

Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. HEK2and its derivatives are used in a wide range of experiments, including signal transduction and protein interaction studies, rapid small-scale protein production, and biopharmaceutical production. HEK 2cell, the Human Embryonic Kidney 2cells was originally derived from human embryonic kidney cells grown in tissue culture.

HEK2Cell Lines Human embryonic kidney 2cells ( HEK 2) are a popular and easy-to-transfect option in cell biology and biotechnology. Transfection efficiencies generally approach 1percent, and the resulting cultures can be used to produce viral vectors, vaccines, and recombinant proteins. M pervanadate for min at 37°, or left untreated. Subcellular fractionation was performed as depicted in Fig.

B) Ten micrograms of each fraction was analyzed by SDS–PAGE and immunoblotting with antibodies specific for phospho-Thr. HEK steht für Human Embryonic Kidney-Zellen, also menschliche embryonale Nierenzellen (HEK). Die HEK-Zelllinie wird auch als HEK-2oder 293-Zellen bezeichnet.

HEK-Zellen werden in der Zellbiologie seit vielen Jahren als vergleichsweise einfach zu handhabende Zelllinie eingesetzt. HEK 2HEK, HEK 2, HEK-2, HEK2Human Embryo Kidney Transformed with sheared human AdDNA. Sensitive to human adenoviruses and adenovirus DNA. Can be used to isolate transformation defective host-range mutants of Adand for titrating human adenoviruses. HEK-Zellen ist die abgekürzte Bezeichnung für Human Embryonic Kidney-Zellen, menschliche embryonale Nierenzellen.

Diese Zelllinie wird auch als HEK-2oder 293-Zellen bezeichnet. T-REx Cell Lines stably express the tetracycline repressor protein (Table 1). They save significant time and effort when using the T-REx System. FreeStyle 293-F cells are adapted to suspension culture in FreeStyle 2Expression Medium.

The HEK2human cell lineage is widely used in cell biology and biotechnology. Here we use whole-genome resequencing of six 2cell lines to study the dynamics of this aneuploid genome in. Since I am using vectors with an SVorigin, I was thinking to use HEK293T cells to perhaps get higher yields.

The HEK cell line has been extensively used as an expression tool for recombinant proteins since it was generated over years ago. Although of epithelial origin, its biochemical machinery is capable of carrying out most of the post-translational folding and processing required to generate functional, mature protein from a wide spectrum of both mammalian and non-mammalian nucleic acids. Author information: (1)Department of Pharmacology, University College London, Gower Street, London, WC1E 6BT, UK. The 293A Cell Line is a subclone of the 2Cell Line and has a relatively flat morphology.

It facilitates the initial production, amplification and titering of replication-incompetent adenovirus. Eine aus Henrietta Lacks Tumor entnommene Gewebeprobe wurde an den Wissenschaftler George Otto Gey übergeben. HEK2cell line: a vehicle for the expression of recombinant proteins.

Der Krebsforscher Gey arbeitete zusammen mit seiner Frau Margaret am Johns Hopkins Hospital bereits seit fast Jahren daran, eine potentiell unsterbliche menschliche Zelllinie zu etablieren, um daran Erkenntnisse zum Wachstumsverhalten von entarteten Zellen studieren zu können.